Yes you can certainly do this with Conditional Formatting, whether or not your data is sorted.
If you have Excel 2007+ select the column then Home ribbon|Conditional Formatting|Highlight Cells Rules|Duplicate Values...|OK
Format|Conditional Formatting...|Condition 1|Formula is:|
If you have an earlier version, select the data (A1:A7 for me) then Format|Conditional Formatting...|Condition 1|Formula is: =COUNTIF(A$1:A$7,A1)>1|Format>>|Fill tab|choose a colour|OK|OK
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